جداسازي باكتريهاي نمك دوست نسبي توليد كننده آنزيمهاي هيدرولاز خارج سلولي و تحمل كننده نمك و بررسي اثر غلظت نمك سديم كلرايد بر توليد آنزيم

Isolation of moderately halophilic indigenous bacterial strains producing salt-tolerant exteracellular hydrolytic enzymes; the effect of NaCl salt on enzyme production

The moderately halophilic bacteria tolerate extensive rang of sodium choloride salt. Intracellular concentration of salt is lower than medium, thus membrane bound proteins and extracellular proteins have halophilic/halotolerant qualities. To isolate the moderately halophilic bacteria, sampling was carried out from Persian Gulf water and different parts of tannery factory. Samples were enriched in selective media for halophils, and bacteria were cultured with streak plate method. In this investigation, 62 bacterial isolates from different parts of tannery factory and 15 strains from Persian Gulf were isolated. The ability of salt-tolerant bacterial isolates were examined for the production of different hydrolytic enzymes. From 42 bacterial strains, isolated from tannery factory, 22 isolates were able to produce some of hydrolytic enzymes. In the case of bacterial strains isolated from Persian Gulf, 8 of 9 total bacterial isolates had capability for producing of hydrolitic enzymes. Results indicated that halophilic bacteria have the high potential to produce different enzymes and their production districted in presence of various concentration of NaCl salt.