مطالعه فعاليت حياتي مخمر ساكارومايسس تثبيت شده در سيليكاژل

Study of Bioactivity of Immobilized Bioactivity Yeast Within Silica-Gel

Introduction: Application of the immobilized cells is in different domains of biotechnology. Silica-gel method can use for immobilization of microorganisms. Aim: In this research the partial bioactivity of immobilized yeast as a eukaryote cell (Saccharomyces cerevisiae) was studied. Material and Methods: Three methods that have been reported in literatures were used for immobilization of cells. Tetra ethyl ortho silicate (TEOS) and Tetra methyl ortho silicate (TMOS) were used as precursors for synthesis of gels. We used different conditions in view of temperature (4°C and 37°C ) and the time of maintenance of immobilized yeast ( 1 ,2 , 3 and 30 days) were investigated. The bioactivity was measured by determination of the CO2 released. Results: If the cell were immobilized by Fennouh and coworkers method the bioactivity percentage for immobilized cells after maintenance at 4°C in 1, 2, 3 and 30 days would be 97, 94, 68 and 63 respectively. Conclusion: After one month of maintenance at 4°C the immobilized cells were appeared maximum bioactivity (63%) by method of Fennouh and coworkers. Other method showed decreasing significantly in bioactivity especially after 30 days.