بهينه سازي شرايط كشت در شيشه گياه Eucalyptus polyanthemos L و بررسي اثر ساليسيك اسيد (SA) بر آن

Optimizing of in vitro tissue culture of Eucalyptus polyanthemos L. and studies effect of salicylic acid (SA)

Introduction: Eucalyptus is production of medical plant which contains essential oils and important in paper pulp and traverse. Salicylic acid is an antioxidant that increases the resistance and organogenesis of plants especially in vitro condition. This substrate such as other antioxidants can destroy free radicals, such as Ros are produce especially in metabolic activities of mitochondria and chloroplasts and have affected on health of cellular structures. Aim: In This study difference concentration of Salicylic acid (0, 0.1, 0.25g.l-l)were investigated on in vitro culture of Eucalyptus polyanthemos with hormonal treated (Kin5mg.l-1,IBA5 mg.1-1 ),(2ip5 mg.1-1, NAA5 mg.1-1) in MS media. Materials and Methods: Each experiment repeats three times on four Petri. This data were analyzed using ANOVA and Duncanʹs test. Results: The final brief results from the statistical analysis were illustrated: Results showed that explants of cotyledon leaves showed that information of callus and root the explants of cotyledon leaves were more suitable than hypocotyls explants. Initial information of callus and organogenesis in control plants (without SA) and the treated plants (in the presence of SA) did not have considerable Difference. Explants of in reached tissue culture were either transported in moistened sterile vermiculite. Stability of explants was (70%).Stability of explants in treated plants (94%) were better than control plants explants (70%). Conclusion: In This study difference concentration of Salicylic acid were investigated on in vitro culture of Eucalyptus polyanthemos with hormonal treated in MS media. Results showed that explants of cotyledon leaves showed that information of callus and root the explants of cotyledon leaves were more suitable than hypocotyls explants. Stability of explants in treated plants was better than control plants explants