كليدواژه :
گروه آموزشي انگل شناسي و قارچ شناسي پزشكي، دانشكده پزشكي، دانشگاه علوم پزشكي كرمان گروه آموزشي انگل شناسي و قارچ شناسي پزشكي , انشكده پزشكي , دانشگاه علوم پزشكي كرمان
چكيده لاتين :
Background and Objectives: Although, more than three hundred thousands types of herbs have been
recognized so far, just ten thousand ones have been tested for their pharmacological specificity. Garlic which is
not used daily has antiseptic, antibacterial, antihelminths and blood pressure reducing specificities. The
objectives of this study are to: 1) study the antifungal activity of the aqueous and methanol extracts of garlic on
Trichophyton mentagrophytes, Microsporum gypseum and Microsporum canis. 2) know the minimum amount of
inhibitory concentration (MIC) of the garlic extracts on these dermatophytes. 3) compare the effects of the
different dilutions of garlic extracts with ketoconazole.
Materials and Methods: In this laboratory study, the fresh bulb of garlic was cleaned, skinned, dried and
powdered. Garlic powder was solved in 80% methanol and distilled water. This yellow solution remained in lab
for four days. After steaming, filtering and concentrating, the concentrated solution was kept inside the pipette in
oven for 48h in 50°C in order to make the dry extract. Methanol and aqueous dilutions (0.625 mg/ml, 1.25 mg/ml
2.5 mg/ml, 5 mg/ml, 10mg/ml and 20 mg/ml), from 200nlg powder of the concentrated extracts of garlic were
prepared separately. Three strains of dermatophytes were cultured on the media which contained different
dilutions.
Results: The MIC of the aqueous and methanol extracts of garlic on T. mentagrophytes and M. canis was the
same; however, the effect of methanol extract on M gypseum was more than effect of the aqueous extract. In
total, extract dilations of 10mg/ml and 20 mg/ml had the highest effects on the growth ofthese dennatophytes.
Conclusion: On the basis of the results of this study, it is clear that garlic plant in either aquatic or methanolic
extract could be effective against Geophilic, Zoophilic & Anthropophilic fungi
