چكيده لاتين :
Background and objectives: Enterococci are members of the normal gut flora of animal and human. They are a leading cause of nosocomial infections. According to transfer of resistant genes between bacteria and human, natural reservoir can affect the distribution of resistant species. The aim of the study was to detect and identify the Antibiotic resistance pattern within isolated Enterococci which was separated from number of Tehran chicken husbandry unites.
Material and methods: 122 isolates of enterococci were selected on Membrane Filter Enterococcus Selective Agar medium, supplemented with vancomycin and identified at the species level by the common biochemical tests and specific genus and species primer. Antibiotic susceptibility test to 6 antibiotics was done by disc diffussion. MIC of vancomycin was also done using broth micro-dillution assay using CLSI recommendations.
Results: Results showed that 26, 45 and 51 isolates were E. faecalis, E. faecium and E. gallinarum respectively. 39, 23, 19, 8, 19, 20 of the isolates were resistant to vancomycin, tetracycline, gentamycin, chloramphenicol, ciprofloxacin and erithromycin respectively. MIC test of the 70% of the isolates were >256 jig/ml.
Conclusion: Although diversity of VRE isolates were restricted to 3 species, but E. faecium had high resistance to broad range of Antibiotics. Results of this study showed the important role of poultry samples as a reservoir of resistance elements. Chicken husbandry precise consideration must be given to sanitary and food industries.
