اثرات توام ميدان الكترومغناطيسي با فركانس بسيار پايين (50 هرتز) و ويتامين A بر رشد و نمو جوانه اندام حركتي جنين موش كوچك آزمايشگاهي

Synergistic effects of vitamin A and extremely low frequency electromagnetic field (50HZ) on limb bud development in Balb/c mouse

Background and aim: Vitamin A is involved in vertebrate morphogenesis, growth, cellular differentiation, and tissue homeostasis. Vitamin A and its metabolites are essential for adequate embryo development. Electromagnetic fields (EMFs) have been used effectively to treat some diseases, such as certain musculoskeletal and chondrogenic disorders. In the present study, we aimed to investigate the synergistic effects of EMFs and Vitamin A on limb bud development in Balb/C mice in vivo. Methods: The Balb/C mice were used as experimental model. After mating, the pregnant mice were divided randomly into 3 groups as control, sham and experimental groups. Control and experimental groups received a single dose injection of Vitamin A (15000IU/Kg) on 10.5th gestational day, interaperitonealy. In addition, animals in experimental group were exposed to EMFs (50 Hz /100 gauss, for 4 h during three days). The animals were killed on the 15.5 th day of gestation and were submitted to caesarian section. The obtained fetuses were examined externally with an analyzed under a stereoscopic microscope and were photographed. Data were analyzed statistically by t-test and ANOVA and Ktuskal- Wallis. Results: Morphological and histological examinations showed significant