تشخيص ويروس بيماري نيوكاسل با استفاده از روش RT-PCR

Diagnosis of Newcastle Disease by reverse transcriptase - polymerase chine reaction (RT-PCR)

Newcastle Disease (ND) is an important problem in poultry industry and rapid diagnosis of its agent is necessary for controlling and protection from this disease. In this study, a molecular test was set up for detecting of Newcastle Disease virus (NDV) by using reverse transcriptase-polymerase chine reaction (RT-PCR) method and comparing with the isolation by embryonated SPF egg. Fourteen suspected samples to ND received from different provinces of Iran and 4 Live Newcastle Disease Vaccines (La Sota, B1, Clone 30, and V4) studied with isolation of the virus by 10 day-old embryonated SPF egg and detection of the virus RT-PCR method by using two primers for the cleavage site of the F gene protein. Newcastle Disease Virus was recognized in all of samples in both methods. Attending the similarity of results, the RT-PCR method could be recommend for detection of NDV for controlling and protection of Newcastle Disease. This test for rapidity, sensitivity and efficiency can be a suitable substitution instead of isolation by embryonated SPF egg method. VetJ.of Islamic. Azad.Univ., Garmsar Branch. 4,2:89-93,2008.