كليدواژه :
آزاتيوپرين , سميت كبدي , موش , ويتامين C
چكيده لاتين :
Azathioprine (AZA) is the widely used immunosuppressive drug for preventing graft rejection and autoimmune disease in medicine and veterinary. However, the therapeutic treatment causes several side effects such as toxicity of bone marrow, gastrointestinal tract, liver, and pancreas. Azathioprine-induced hepatitis was found to be associated with oxidative damage. Vitamin C is a water-soluble vitamin. The major function of vitamin C is acting as a natural antioxidant by scavenging free radicals. Besides, vitamin C is important for preventing peroxidation of unsaturated membrane fatty acids. Noticing to the kind of Azathioprine-induced damage in Liver tissue, we decided to examine the protective effect of Vitamin C against Azathiprine-induced toxicity. In this study, 40 male rats ( Wistar_breed) were divided to 4 groups (each group contained 10 rats). Group 1 was control group and only it was given normal salin. Group 2 and 3 got Vitamin C everyday orally for 7 days. Group 4 was administrated with normal salin instead of Vitamin C in same condition with group 2 and 3. In7th day, group 3 and 4 were administrated with single dose of AZA, 15 mg/kg (IP). After 24 hours, we took blood and tissue samples ,then they were examined for biochemical and pathological examination. This study may showed that Azathioprine-induced damage on liver in group 3 is less than group 4 and the function of organ in group 3 was nearly as the same as with control group. The results demonstrated that vitamin C decreased Azathioprine-induced hepatotoxicity in rats. Noticing to the importance of Azathioprine-induced damage, and the results of this study, co-administration of Azathioprine with vitamin C is suggested because of the decreasing the toxicity of this drug. Vet.J.of Islamic.Azad.Univ., Garmsar Branch. 4,1:39-44,2008.
