كليدواژه :
آكريل آميد , نوروپاتي , رنگ آميزي نقره , نوروتوكسيسيتي
چكيده لاتين :
Acrylamide (ACR) is a water soluble, vinyl monomer that has multiple chemical and industrial applications, the polymer is nonneurotoxic, exposure of humans and laboratory animals to monomeric ACR produces ataxia, skeletal muscle weakness, and weight loss. This study was coducted to determine the neurotoxic and axonopathic effects of different doses of ACR on cerebellum of rat. To evaluate this hypothesis in cerebellum, the amino cupric - silver stain method of de Olmos was used to define the spatial characteristic of nerve somal, dendritic, axonal and terminal degeneration in cerebellar nuclei and regions. For this purpose 55 adult male rats (Wistar, approximately 250 g) were selected and housed in polycarbonate boxes as two per each. Randomly assigned groups of rats (10 rats per exposure group., total 5 exposure groups as A,B,C,D,E,) were exposed to 0.5, 5, 50,100 and 500 mg/kg per day x lldays i.p. respectively. The remaining 5 rats were housed in group F, as control group. Control rats received daily i.p. injections of 0.9% saline ( 3ml/kg). As indices of developing neurotoxicity, weight gain, gait scores and landing hindlimb foot splay were determined. After 11 days, two rats for silver stain, were randomly selected, dissected and proper samples were collected from cerebellum. Results did show no neuropathological behavior in groups A, B and F, whereas sever neurotoxicity was observed in group C [decreased weight gain of 19.90% and (±SEM) gait score = 3.77±0.14]. Rats in groups D and E died within 1-2 hours due to sever toxemia. In histopathological studies based on de Olmos amino-cupric silver staining technique no argyrophilic neurons or processes were observed in stained sections obtained from cerebellum of rats belong to groups A, B and F, while moderate to severe argyrophilic changes were observed in different nuclei and regions of stained sections obtained from cerebellum of rats belong to group C. Vet.J.of Islamic.Azad.Univ., Garmsar Branch. 3,4:163-172,2007.
