بررسي تأثير عصاره گياه فلوميس توبروزا در التيام زخم تجربي پوست در خرگوش، مطالعه مورفومتريك و هيستوپاتولوژيك

Evaluation of the effect of Phlomis tuberosea herbal extract on healing of experimental skin wound in the rabbit, morphometric & histopathologic study

Nowadays faster healing of surgical wounds for decreasing of wound complications is more challenging to researchers. Phenyl ethanoide, phlavonoide and iridoide, as more effective substances in improvement of wound healing, are existent in phlomis tuberosea, abundantly. The aim of the present study was to evaluate the healing effects of herbal extract of phlomis tuberosea on skin wounds in rabbits. Male Albino rabbits were randomly assigned into four groups of five animals each. A full thickness surgical wound (2.5x2.5 cm) was made on the back skin of any rabbits with scalpel. Groups 1 was treated with extract administered daily for one week intramuscularly in a dose of 100 mg/kg (high dose) which followed as every other days by next three weeks. Groups 2 was treated with extract in a dose of 50 mg/kg (low dose), similarly. Groups 3 received DMSO and Group 4 (control) was treated with ISS, in this manner. Histopathologic examination was performed on biopsy tissue specimens in days 0th, 3rd, 7th, 14th, 28th of the experiment and wound healing was scored considering four temporal phases of repair including 1-Hemostasis, 2-Acute inflammation, 3-Proliferation (Granulation), 4-Remodeling (Maturation, Contraction) and 5-Re-epithelialization. Morphometric study of the wounds was carried out using transparent and graph paper, simultaneously. In comparison to the control group, there were significant differences of wound healing in skin of the rabbits between the experimental groups and from this view point, the effect of low dose extract was more prominent. The main overall finding from this study is that phlomis tuberosea herbal extract may be of therapeutic benefit in the healing of full thickness surgical wounds. Vet.J.of Islamic.A7jad.Univ., Garmsar Branch. 3,4:185-194,2007.