ارزيابي مقاومت كلون هاي اميدبخش سيب زميني در برابر ويروس هاي PVX، PVY و PVA در گلخانه

Evaluation of Resistance of Potato Promising Clones to PVX, PVY and PVA in Greenhouse

The reaction of sixteen potato-promising clones to PVX, PVA and PVY was evaluated in greenhouse condition. At first, viral infection of each clone was examined using DAS-ELISA. Infected clones were treated by electrotherapy technique to achieve virus free clones. Single nodes of healthy plants were cultured on MS medium. After growing, plantlets were transferred to greenhouse and minitubers harvested about two months later were used as experimental materials. Three week old potato plants were inoculated mechanically with tobacco plants extracts infected to PVX, PVY and PVA. After 30 days, the result of DAS-ELISA of each plant was scored. Data analysis showed that the reaction of clones to the three viruses were significantly different at 1% level. However, the difference among plantlets in each replicate was insignificant. Mean comparison of clonesʹ ELISA absorbance values test indicated that clones 397015-1 and 397015-13 were resistant to all the three viruses and clone 397008-2 was susceptible to three viruses. Other clones were resistant or susceptible to one or two viruses.