بررسي تغييرات پروتييني در برگ گياه Lycopersicon esculentum cv.Isfahani تحت تنش شوري با استفاده از تكنيك الكتروفورز دو بعدي

Study of protein changes in tomato (Lycopersicon esculentum) under salt stress using two dimensional electrophoresis

Plants use complicated mechanisms to respond stress, using proteins involve in signaling pathways for detoxification. The results of SDS-PAGE in the present study showed down regulation of proteins with 20, 26 and 50 KDa in stem-leaf and 30 and 75 KDa in roots in tomato cultivar Isfahani. Two-dimensional electrophoresis of proteins in leaf extract under salt stress showed differences in intensity of expression of some protein spots compare to the control. These spot proteins can be categorized into 2 groups: 1- protein spots up regulated or down regulated under salt stress 2- protein spots which appeared or disappeared under salt stress. It can be concluded that salt stress directly or indirectly affects on metabolism.