نگهداري بذر كيكم (Acer monspessulanum) در شرايط فراسرد

Cryopreservation of Acer monspessulanum seeds

Montpellier Maple (Acer monspessulanum) is one of the broad leaf species grows in semi-arid and cold climate of I.R. Iran. Preservation of this wild woody species has a great importance in natural resource point of view. Cryopreservation technique is used to preserve seed and vegetative organs under -196 °C conditions. In the mentioned temperature, all of the metabolic and physiological activities of the seed or organ almost is stopped. As the result, seed and organ can survive for a long period of time. In order to preserve A. monspessulanum seed under -196 °C condition, three pre-cry©preservation treatments, PVS2 solution, Desiccation and 30% Glycerol were applied. The treated seeds were transferred into Liquid Nitrogen (LN) and stored for 7 and 30 days respectively. After finishing storage period, the seeds were removed from the LN, imposed to heat shock and transferred between wet-papers in germination boxes. The cryogenic seeds together with control seeds were incubated at +4 °C for 105 days to germinate. Mean germination percentage of the cryopreserved seeds was 19.18% and, there were no significant differences between the control and cryopreserved seeds. No adverse effects or abnormalities were observed on seedlings developed from cryopreserved seeds. The results showed that, the long-term preservation of the species, seed in -196 °C using suitable pre-cryopreservation treatment is possible.