چكيده لاتين :
Aims: The Botulinum toxin is known as the most potent toxin. In Iranian population, the type E is prevalent in individuals being poisoned by using contaminated foods (especially canned fish). So, the purpose of this study was to produce Clostridium botulinum type E antitoxin for treatment of food poisoning.Methods: For immunization of lab animals (chicken, mouse and rabbit),at first, the toxoid was injected with Freundʹs complete adjuvant either subcutaneously or intramuscularly and in the first to third booster injection ,it was injected with Freundʹs incomplete adjuvant. At the end or intervals of injections, blood sampling was done and the produced antibodyʹs titer was measured using the enzyme-linked immunosorbent assay or ELISA method.
Results: After each booster injection, a considerable increase in serum antibody titer of lab animals was seen. The titer of Clostridium botulinum type E toxin antibodies in the serum of chicken, rabbit and mouse increased to more than 1:3200. After purification of antibody, less than 0.156 microgram of the purified mouse antibody and less than 0.312 microgram of rabbit purified antibody were able to detect the toxin.
Conclusion: After the injection of toxoid, antibody increase in the body of lab animal. Production of type E specific antitoxin based on reaction between antigen and antibody is necessary for treatment of poisoning.
