اندازه گيري ميزان آفلاتوكسين M1 در شيرهاي پاستوريزه

Measuring the amount of Ml Aflatoxin in pasteurized milks

Aims: This study was conducted to investigate the pasteurized milks contamination with Ml Aflatoxin (AFM1) and comparison of the products of two main factories that provide one of Tehran Universities dairy needs, in terms of contaminating with this mycotoxin.Materials & Methods: Fifty milk samples produced by two dairy factories were randomly selected during two cold (winter 2008) and warm (summer 2009) seasons and their AFM1 concentration was determined by a competitive Enzyme-Linked Immuno Sorbent Assay (ELISA) method. The mean differences were analyzed using student T-test in software INSTATA environment. Results: 84% of the examined milk samples were contaminated with AFM1 by measurable amounts. Mean of the mycotoxin concentration in whole samples was 20.7+14.6 ng/1 ranging from 0 to 63.4 ng/1. AFM1 contamination was higher than Iran national standard (50 ng/1) only in two (4%) of the milk samples. The mean concentration of AFM1 was higher in no. 1 companyʹs products (25.3+15.7) in comparison with no.2 company (15.4+11.6; p<0.01). The no. 1 companyʹs winter products (31.2+15 ng/1) were more contaminated in contrasting with its summer products (18.5+14.2 ng/1; p<0.05). Conclusions: High prevalence of AFM1 contamination in pasteurized milk samples is worrying and notifies the necessity of preventing measures to reduce entrance of B1 Aflatoxin to dairy animalsʹ feed and more controlling measures on milk distribution