بررسي منشاء شناسي مولكولي (phylogenetic) ويروس نقص ايمني (HIV) در مشهد- ايران

A Molecular Phylogenetic Study on Human Immunodeficiency Virus in Mashad, Iran

Human immunodeficiency virus (HIV) which is responsible for acquired immunodeficiency syndrome (AIDS) is one of the most important Retro viruses in the world. At the beginning of the third millennium, policy makers consider AIDS as one of the most mortal, social and health problems of human beings. Identifying the source and integral molecule of virus can be useful for the scholarsʹ future studies .The aim of this study is a molecular assessment and phylogenetic | Materials and methods: The study of Molecular Phylogenetic was done on a group of Iranian IV drug abusers, as volunteers, in Mashhad University of Medical Sciences at the end of the year 2005. The blood samples of 12 persons were identified positive for HIY, as documented by immunological methods (i.e. ELISA, Western Blot) as well as counting the infected CD4+ T lymphocytes. Phylogenetic trees were made, using adjacent connection method with Tree View (a soft ware), used for drawing family trees. Genetic gaps were also calculated by two parametrical methods (Kimuras). | Results: All HIV samples found in Mashad are phylogeneticaly classified into group and subgroup A reference sequences in gag sub-genome zones and at the same rate env which there was no close phylogenetic relationship between reference sequences of A subgroup and circulating recombinant forms based on A. Mashhad samples, classified in a separate branch, indicated a strong phylogenetic relationship and introduced a recent HI V-l infection in the society, with limited genetic development of circulating virus. Phylogenetic Analysis revealed a close relationship between the viruses identified in Mashhad and in African sub-Saharan area. £[ Conclusion: The epidemic infection of HIV-1 influences a high-risk group in Iran with predominance of IV drug abusers (the predominant mode of transmition). The virus is derived from subgroup A viruses. Besides, a strong phylogenetic relationship between the separated ones indicates the probability that the virus has more likely been transmitted from Africa, especially Uganda, than from the neighboring countries. However, Iranʹs neighboring countries remain as the main sources of A Subgroup species which are common all over Europe.