بررسي نقش سايتوكاين ها در عفونت بروسلوز، آرتريت و كمر درد بروسلايي

Role of Cytokines in Patients with Arthritis and Low Back Pain Brucellosis

Introduction Cytokines are secreted as the molecular products of different cells in particular T-Lymphocyte which contribute in the processes of immune responses. Therefore, the study may provide valuable information regarding the function of cell-mediated immunity ( CMI) in this disease. Materials and Methods This study was performed in the medical centers of Mashhad University of Medical Science. 40 Patients with brucellosis and 40 normal samples took part in this investigation. Diagnosis of the disease was based on case history clinical examination and serological tests. Results The levels of TNF-a, IL-2, IFN-y, IL-4, and IL-10 in serum were measured using ELISA method. The levels of the random variables IL-2 and IL-10 were tested by Kolmogrov Smimov test and shown to be normal (p<0.05). Other cytokines including IL-4, IFN-y, and TNF-a of patients serum were not normal. The results indicated that the levels of IL-10 in the sera of patients had increased (p<0.001). IFN-y level showed it has increased in patients (p<0.05). The level of IL-2 and IL-4 and TNF-a in the sera of patients did not show any appreciable difference with those of normal controls (p>0.1). Kruskal-Wallis test showed a significant difference in IFN-y in the two groups. Conclusion Our results w ould suggest a definite role of IFN-y, and IL-10 in sera of patients with arthritis and low bake pain brucellosis.