بررسي اثرات پروتيين هاي سيتوپلاسمي كانديدا آلبيكنس بر روي الگوي ترشحي Th1 و Th2 در موش Balb/C

Cytoplasmic Proteins Effects of Candida Albicans Upon Secreting Profile of Th1 and Th2 in Balb/c Mice

Background: The incidence of mortality due to mycoses, particularly those caused by opportunistic fungi, has shown a marked increase in recent years. This happens especially in patients whose immune defense mechanisms is compromised by antibiotics, immunosuppressive, therapy, or severe underlying disease. In this study, the effects of cytoplasmic proteins of Candida albicans on lymphocytes were examined. Methods: Candida Albicans (Atcc 1023) was cultured in GYEP medium. Cells harvested by centeri-fugations were disrupted with glass beads. Disrupted yeast cells were centrifuged at l00ʹ000 x g for 1 hour and supernatant was taken as cytoplasmic extract. SDS -PAGE was done to determine molecular weight. In order to further purify the cytoplasmic extract, gel filtration (G200) was used. Mice were divided in five groups: two groups were sensitized with active and inactive cells (both groups with adjuvant) and the other three groups were sensitized with complete adjuvant, incomplete adjuvant and physiologic serum. After seven days of the last injection, spleens were removed and lymphocytes were isolated. MTT assay was used in order to measure lymphocytes proliferation. IL4 and IFN-y cytokines were thereafter measured by ELISA. Findings: Among five groups of mice, antigens in groups 1 and 2 had the highest stimulation index and group 2 showed more stimulation. Studiy of cytokine profile showed that Thl pattern in cell-mediated immune responses to the cytoplasmic proteins of Candida albicans. Conclusion: According to the results, cytoplasmic proteins modulate immune responses as inducers of lymphocyte proliferation and cytokine produce in vitro. So it can be suggested as a model for vaccine. Immunotheapy with interacellular Candida constituents as cytoplasmic proteins will help to inhibit the emergence of resistant Candida strains and to treat fungul infections caused by chemothera-peutics after infection.