ارزيابي تنوع ژنتيكي در نمونه هاي دو گونه جو (L. H. vulgare وH. spontaneum L. ) با استفاده از نشانگرهاي ريزماهواره

Assessment of genetic diversity in two accessions of barely species (H. vulgare L. and H. spontaneum L.) using SSR markers

Genetic diversity of 73 accessions of two barely species (//. vulgare L. and H. spontaneum L.) was evaluated using 15 SSR pair primers. Patterns were scored by 0 (band abscence) and 1 (band presence). Dendrogeram was constructed using dice similarity coefficient and UPGMA algorithm by software NTYSYS 2.02. Principle coordinate analysis was also performed. The highest number of polymorphic alleles in H. vulgare L. accessions were observed in gene loci Bmac 0316 and Hvm40 with eight alleles in each. The lowest number of polymorphic alleles in H. vulgare L. accessions were observed in gene locus Hvm70 with two alleles. Moreover, the highest number of polymorphic alleles in H, spontaneum L. were observed in Hvm40 locus with 9 allels, while the lowest obtained in Hvm70 and HvmloH1A with two alleles, respectively. The highest PIC value in H. vulgare L. was 0.8 (gene loci Bmac0040) and the lowest 0.19 (gene loci Bmag0603). While, the highest and the lowest value of PIC in H. spontaneum L. ranged from 0.43-0.91. Cluster analysis using Dice similarity method and UPGMA algorithm, grouped all accessions in three main groups. Group one included populations from both of H. vulgare L. and H. spontaneum L. species collected from Iran, while groups two and three included accessions of H. spontaneum L. and H vulgare L., respectively. Results showed that SSR markers can be apllied as reliable tool to evaluate genetic diversity between and within H. vulgare L. and H. spontaneum L. species. Loci HvmLoH1A and Ebmac0415 in H. spontaneum L. and loci Bmac0306 and Bamc0040 in H. vulgare L. can be used to estimate genetic diversity in these two barely species.