بررسي توانايي كلونيزاسيون لاكتوباسيلهاي جدا شده از پنير محلي و اتصال به سلول هاي پوششي لوله گوارش انسان ،لاين سلولي Caco2

Evaluation of colonization ability of lactobacilli isolated from traditional cheese and adherence to epithelial cells of human GI tract, Caco-2 cell line

The aim of this study was to asses5 colonization ability of 7 lactobacilli strain isolated from traditional cheese in GI1 trace. So resistance to acidic pH, bile salt and ability to adhere Caco- 2 cell line was assessed in vitro. 2 hour acidic treatments lead to 0-4 log reduction of tested strains. Isolate B1D2 was fully resistance and no reduction in population observed after acid treatment. Bile salt cause growth delay between 25-180 min for all strains and BHlwith D= 25 ± 4.80 min was the most resistance strain. Ability to adhere Caco-2 cell line was strain depended and GI2 and SL2 were less and most adherent strain respectively. In conclusion evaluation of these 3 factor in vitro indicated that SM3, BD2, SMI, SL2 isolated from Semnan and Babol cheese have colonization ability.