تداخل اثر عصاره آبي گياه شاهتره و كلرامبوسيل بر اسپرماتوژنز در موش صحرايي

Interaction Between Fumaria parviflura Aqueous Extract and Cholorambocil on Spermatogenesis in Rats

Introduction: Fumaria parviflura belongs to the Fumariacea family. Medicinal properties of Fumaria derived from green fork-like leaves and violet flowers that the antioxidant effects of these items has been recently noticed. Cholorambocil (CHL) is an effective substance of the leukeran that is used against the human neoplasm disease. It is demonstrated that CHL in specific dosage has severe destructive effects on the testis tissue. The aim of this study is to investigate the effect site of CHL in testis tissue and protective role of the Fumaria aqueous extract, against. Materials and Methods: Wistar male rats divided into 3 groups: control, control2 , experimental 1,2,3,4 (n=7). The control group received distilled water, the c0ntrol2 group received distilled 10 mg/kg w.b. CHL , the experimental groupsl, received distilled 150 mg/kg w.b Fumaria aqueos extract and the experimental groups 2,3,4 received 150,250,350 mg/kg w.b CHL and 150, 250 ,350 mg/kg w.b Fumaria aqueous extract and lOmg/kg.wb CHL through oral gavage respectively. After 15 days rats were anesthesized and blood test performed. Rats sacrificed and the Testis were removed, the volume and weight of the testis were measured and the tissues were fixed. The preparation of Tissue sectioning accomplished by H&E staining. Sertoli and spermatogenic cells counted with light microscope. Results and Discussion: Statistical analyzes showed that CHL has significantly reduced cell groups specially primary spermatocyte and Fumaria aqueous extract has significantly restrained this effect.