عنوان به زبان ديگر :
Effect of Sampling Time, Explant Type, Culture Orientation and Antioxidant Type on in-vitro Explant Establishment and Growth of Persian Walnut
پديد آورندگان :
ياري، محمد باقر نويسنده دانشجوي كارشناسي ارشد yari, mohammad bagher , غلامي ، منصور 1332 نويسنده كشاورزي و دامپزشكي gholami, mansour , اثني عشري ، محمود نويسنده ASNA ASHARI, mahmood
چكيده فارسي :
در اين تحقيق، چهار آزمايش مجزا جهت مطالعه عوامل موثر بر استقرار و رشد ريزنمونه هاي دو ژنوتيپ گردو Z60 و Z63 جمع‌آوري شده از ايستگاه تحقيقات گردوي تويسركان اجرا گرديد. آزمايش ها شامل بررسي پنج زمان نمونه‌گيري، چهار نوع ريزنمونه، سه حالت قرار دهي ريزنمونه روي محيط كشت و شش نوع ماده آنتي‌اكسيدان بودند. در هر دو ژنوتيپ، ريزنمونه هاي مريستم انتهايي و تك گره كه در ارديبهشت‌ماه گرفته شدند به دليل داشتن نقطه فعال رشد و نيز شرايط فيزيولوژيكي مناسب بيشترين رشد و استقرار را دارا بودند و نسبت به ساير ريزنمونه ها از اين نظر اختلاف معني داري را نشان دادند. همچنين ريزنمونه هايي كه به صورت عمودي و به سمت بالا روي محيط كشت قرار داده شدند رشد بهتر و بيشتري داشتند. در ژنوتيپ Z63 نتايج نشان داد كه پلي‌وينيل پيروليدون و فلوروگلوسينول موجب افزايش استقرار ريزنمونه ها و كاهش تعداد ريزنمونه هاي قهوه اي شده گرديدند و علاوه بر آن باعث افزايش معني دار در وزن تر و قطر كالوس نسبت به ساير تيمار ها شدند. در ژنوتيپ Z60 سيستيين اثر معني داري در افزايش استقرار ريزنمونه ها، كاهش تعداد ريزنمونه هاي قهوه اي شده و افزايش وزن تر كالوس داشت.
چكيده لاتين :
Four separate experiments were conducted to study the effects of factors controlling the establishment of explants from two walnut genotypes Z60 and Z63 (Damavand). Factors were explant type, sampling time, explant orientation on the growth medium and antioxidants type. The explants were collected from the collection Garden of Tooyserkan Walnuts Research Station. Four explant types (apical meristem, single node, petiole and leaf), five sampling times (April, May, June, July and August), three modes of explant orientation on growth media (vertical, inverted vertical, and horizontal) as well as six antioxidant agents (ascorbic acid, citric acid, cysteine, asparagine, polyvinyl pyrolidone, and fluroglucinol) were studied. Culture Medium was DKW supplemented with 0.2 mg IB A. For either one of the genotypes, apical meristem and single node explants were collected in April and May. Due to the existence of plant growth hormones in meristematic tissue, the highest growth and establishment was observed for apical meristem type of explants, and with significant differences as compared with the other types of explants. Also explants oriented vertically and upwards on the growth medium, due to normal stem polarity direction and probably better absorption and transfer of nutrients, had greater growth, exhibiting significant differences in comparison with other treatments. As for Z63 genotype, after a passage of six weeks time, results indicated that Polyvinyl Pirolidone, and Fluroglucinol increased the rate of explants establishment, reduced the browning of explants and significantly increased callus fresh weight and diameter. For Z60 genotype, Cysteine antioxidant had significant effects on increasing the explantsʹ establishment, reducing the rate of browning as well as increasing callus fresh weight.
