توليد گلوتاميك اسيد از ضايعات خرما با بكارگيري كرينه باكتريوم گلوتاميكوم به روش تخمير غوطه وري

Glutamic Acid Production from Date Wastes by Corynebacterium glutamicum under Submerged Fermentation

Iran is one of the largest date producer countries and approximately 50% of date production is wasted annually. The goal of this study is to produce glutamic acid from date waste using Corynebacterium glutamicum CECT690 by submerged fermentation. In this study the effect of five variables including inoculum size, substrate concentration, penicillin amount, phosphate amount and inoculum age in five levels using response surface methodology (RSM) has been studied. The greatest value of glutamic acid production was obtained 26.67 mg/mL. The variable optimum contents of inoculum size, substrate concentration, penicillin amount, phosphate amount and inoculum age obtained 2%v/v, 25%w/v, lU/mL, 4g/L and l0h, respectively. The model predicted 39.32 mg/mL for maximize concentration of glutamic acid. Experimental results in predicted optimum conditions confirmed the model validation.