تكثير درون شيشه اي چهار ژنوتيپ پاكوتاه گزينش شده محلب

Micropropagation of four selected dwarf mahaleb (Prunus mahaleb L.) genotypes

used as a principal rootstocks for sweet and sour cherries in Iran. This study was conducted for optimization of micropropagation in four selected dwarf mahaleb (.Prunus mahaleb L.) genotypes. The influences of type and concentration of plant growth regulators on bud explants of mahaleb were studied. The statistical design was factorial adopted completely randomized design with 5 replicate. Explants were taken in end of Winter. For disinfection, they were dipped in 70% ethanol for 30 s followed by immersion in 0.1, 0.2 or 0.3% of mercuric chloride for 2 or 4 minutes. Application of 0.2% mercuric chloride solution for 2 minute was the most effective way for surface sterilization of the explants. Satisfactory stabilization of aseptic culture was achieved in 40-60 days. After three subcultures of each 21 days, on different proliferating media. The highest rooting percentage was obtained with MS supplemented with lmglʹ1 BAP and lmglʹ1 GA3 during proliferation. Proliferation was greatly influenced by genotypes. The best results were obtained in MS supplemented with 0.8mglʹ1 IBA. The rooting percentage of four selected dwarf mahaleb ranged from 1.5 to 43.2%, depending on genotype and IBA concentration in the rooting media.