استخراج و اندازه گيري تروپان آلكالوييدهاي -L هيوسيامين و (-)- اسكوپولامين (هيوسين) از اندام هاي مختلف .Hyoscyamus reticulates L و .Hyoscyamus pusillus L

Extraction and determination of tropane alkaloids, hyoscyamine and scopolamine, from different parts of Hyoscyamus reticulatus L. and Hyoscyamus pusillus L

The alkaloids of solanaceae family have very medicinal usages. From these hyoscyamine and scopolamine are used as antispasmodic and mydriatic medicines. Hyoscyamus from solanaceae family with different species has specific medicinal value. In this research, Hyoscyamus reticulatus L. and Hyoscyamus pusillus L. are investigated for tropane alkaloids value. The plant material of H. reticulatus were collected during the end of flowering stage and beginning of fruit formation, from two regions of Tehran - Latyan dam and Ghazvin- Barajin and the plant material of H. pusillus were collected at the flowering stage from Qom. After extraction of tropane alkaloids from different parts of plants, distinctly, the tropane alkaloids value of L-hyoscyamine and (-)-scopolamine were assayed by HPLC. The results showed that L- hyoscyamine and (-)- scopolamine content varied in different parts of plants. L- hyoscyamine in all organs of H. reticulatus in two regions and (-)- scopolamine in different parts of H. pusillus were dominant alkaloids. Futthermore, the results of determination of tropane alkaloids value showed that, in Tehran region there was higher tropane alkaloids than Ghazvin.