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The Influence of extender solution in different dilution ratios accompanying antibiotic on short-term storage and sperm motility of ship sturgeon (Acipenser nudiventris Lovetzky, 1828) semen

The short-term storage of the endangered ship sturgeon (Acipenser nudiventris) semen and effect of extender solution in 8 dilution ratios,5 concentrations antibiotics (penicillin and streptomycin) on motility duration spermatozoa were studied. The fresh semen (n=3) was mixed with extender solution at a 0.5, 1, 1.5, 2, 2.5, 3, 3.5 and 4 ratios. For preventing bacterial growth, 5000 units of penicillin + 5mg streptomycin/ml, (4000+4, 3000+3, 2000+2, 1000+1 and 0) were added to the stored semen. Samples of stored semen (in the refrigerator) were analyzed for sperm motility at 1, 3, 9, 14, 21, and 28 day after collection. The extender solution significantly(p<0.05) prolonged the sperm motility for up 21 day at 4oC. Sperm motility from Ship Sturgeon deteriorated within 6 hours after collection when stored at lab temperature (20-22 oC). Undiluted semen from this specis lost its viability within 5 day of storage in the refrigerator. In this study,significant difference is observed in the motility duration between dilution ratio (p<0.01). During semen storage, maximal and minimal motility duration were observed in 1:1 and 1:4 dilution rates respectively. The motility duration was similar in all antibiotic concentrations at 1 and 3 day after semen storage in 4°c (p>0.05) but significant different was observed at other days(p<0.05).Results finally indicated that use the extender solution in 1:1 dilution ratio and use of antibiotic in low concentration(l000Iu P+lmg S and 2000IU P+2mg S) prolonged the spermatozoa viability in short-term storage of Ship Sturgeon semen.