اثر تنش موليبدن بر مقدار آنتوسيانين، پروتئين، مالون دآلدئيد و فعاليت پراكسيداز در دو واريته گياه شيرين بيان Glycyrrhiza glabra L.)L) در شرايط كشت درون شيشه

Effect of molybdenum stress on anthocyanin, protein, malondealdehyde content and peroxidase activity in two varieties of licorice (Glycyrrhiza glabra) in vitro

Molybdenum is a heavy metal that its high concentration is toxic in plants. In this study, toxic effect of Na2Mo04.2H20 on licorice (Glycyrrhiza glabra L.) var.glabra and var.glandulifera callus was investigated. Calli cultivated on the MS medium containing 0, 250, 500, l000mM sodium molybdate for two weeks. Then calluses were used for biochemical analysis. In molybdenum treatments significantly increased content of anthocyanin. Peroxidase activity significantly increased in var. glandulifera in molybdenum treatments, but in var.glabra increase of activity only was observed in 250,1000 mM molybdenum. In var.glabra, molybdenum treatments increased protein content and 500 mM molybdenum caused the highest protein content whereas in var.glandulifera the quantitive of protein wasnʹt change. Malondealdehyde content increased in high level of molybdenum. Therefore, molybdenum stress increased anthocyanin content and peroxidase activity in this plant as well as toxicity symptoms appeared as peroxidation lipid in high concentration of molybdenum.