بررسي تنوع ژنتيكي ارقام انگور استان آذربايجان غربي با استفاده از نشان گر مولكولي AFLP

Genetic diversity assessment of West Azerbaijan grape cultivars by AFLP marker

To assess the genetic diversity and relationships of 61 Iranian cultivated grapevine ampelography along with molecular analyses with AFLP markers were used. A total of 459 scorable fragments across all the accessions analyzed were detected. Among these 185 (40.3%) were polymorphic. Values of genetic diversity calculated by the Nei and Li index, ranged from 0-0.3. Cluster analysis classified all tested cultivars into four groups. Based on AFLP analysis, high genetic similarity was found in several genotypes such as ʹKeshmeshi Sefidʹ and ʹBidane Qermezʹ, Tabarze Sefidʹ and ʹTabarze Qermezʹ, ʹQara Melhiʹ and ʹQara Shiraʹ. Also Occurrence of synonyms was proved in ʹFakhriʹ, ʹDizmariʹ, ʹMoseliʹ and ʹSaghal Solian\ Results of AFLP analysis showed the misnaming of ʹAlhaghiʹ and ʹShahroudiʹ as well as ʹKhalili Sefidʹ and ʹKhalili Qermezʹ that confirmed by morphological data. In this research, AFLP analysis revealed differences in clone level in ʹKeshmeshi Sefidʹ, ʹBidane Qermezʹ, ʹTabarze Sefidʹ and ʹTabarze Qermezʹ but couldnʹt detect any polymorphism between ʹRashaʹ and ʹKhoshnavʹ.