بررسي اثر بازدارندگي Lactobacillus plantarum بر رشد Aspergillus flavus و كاهش آفلاتوكسين B1 در شرايط آزمايشگاهي

In vitro investigation of Aspergillus flavus inhibition growth and Aflatoxin B1 uptake by Lactobacillus plantarum

Laboratory studies assessed the binding of aflatoxin B1 (AFB1) and its adsorption by many strains of lactic acid bacteria which werw used in foods. In this study the binding ability of AFB1 ? a main endoded Lactobacillus plantarum (PTCC 1058) was studied. The 0.5 ppm AFB, solution was inoculated at 37°c for 1, 24, 48, 90 hours and the AFB1 residues were quantitively in supernatant by HPLC. Results showed this strain removed 45% of AFB 1 from solution within lh and 100% of it in 90h. In death phase, this bacteria removed only 31% of AFB1 from solutions after 1 and 24h, but this value reduced to 15% after 48 and 90h incubation periods (P<0.05). The stabilities of the amount of AFB, to bacterium cell wall were evaluated by usign organic solvent (chloroform) and the result indicates no AFB1 extraction by solvent. The low amount aflatoxin production by three different methods represented high level of fungus growth suppression by lactobacillus.