بررسي اثر ضدعفوني كننده ها بر بيوفيلم باكتري هاي مختلف

The Assessment of Disinfectants on Various Bacterial Biofilms

Biofilm is a functional consortium of microorganisms attached to the surface and is embedded in the extracellular polymeric substances (EPS) produced by the microorganisms. The biofilm consists of microbial cell clusters with a network of internal channels or voids in the extracellular polysaccharide and glycoprotein matrix (EPS). Biofilms, due to special structure and EPS, are more resistant to antimicrobial agents. Thus control measurement such as heating, drying and detergents can not eliminate them. Biofilms even with cleaning and disinfecting methods can remain on various surfaces which may assist the survival of pathogenic and spoilage bacteria in the food processing environment, a contributing factor in foodborne disease outbreaks. In this study biofilm elimination potentional and bactericidal effect of current food industry and clinical disinfectants on biofilm of pathogenic bacteria such as Salmonella enteritidis, Listeria monocytogenes and Staphylococcus aureus and environmental bacteria from meat processing factory were assessed by microtiter plate method. Biofilm elimination potentional and bactericidal effect were assessed by crystal violet and TTC stain, respectively. Results indicated that bacterial biofilms are resistant to disinfectants. However, Hypochlorite Sodium, Propanol and Peroxide Hydrogen had the most Biofilm elimination and Glutaraldehyde had the most bactericidal effect on biofilms. Thus, efficiency of disinfectants based on strain, structure and environmental condition is different. This indicates the importance of appropriate choice of disinfectants in industries and hygiene centers for prevention and control of biofilm formation.