ارتباط بين پلي مورفيسم ژن IL 1RA با بيماري تشنج ناشي از تب در كودكان شهركرد

Assosiation study between IL1RA gene polymorphism with febrile convulsion in Shahrekord children

Background and aims: Now a days, fever and seizure are the most reasons for admitting children in hospitals. Due to influence of genetic factors, some children undergo to the fever less than others. In addition, recent studeis have shown a positive correlation between family history for febrile convulsion (type and age of onset in child) and predisposition to this disease. Therefore, this study was performed to study the assosiation between IL1RA gene polymorphism and predisposition to the disease. Methods: In this case-control study, 100 patients affected by febrile convulsion who were referred to pediateric and emergency department of Hajar hospital were selected as case group and the control group was consisted of 130 healthy children. Peripheral blood sample (1.5 ml) was collected from the patients and DNA was extracted by standard phenol-chloroform method. Classic PCR was performed using one set primers designed for Inter Luekine 1 receptor antagonist and in the next step PCR products were analysed by PAGE (Poly Acryle Amid Gele Electrophoresis) and finally results were analysed by comparision of segments size. Results: The average age of the patient group was 3.4±1.4 years and the average age of the control group was 3.4±1.2 years old. A positive history for febrile convulsion was detected for 44 cases of the patient group. The genotypic frequencies of the IL1RA gene allele 1 and 2 in the patient group were 56% and 10%, respectively and for the control group were 55.4% and 6.9% respectively. Considering P=0.93 for allele 1 and P=0.401 for allele 2, no significance difference was found between two groups. Conclusion: Based on the Chi square test, there was no correlation between IL1RA polymorphism and predisposition to disease.