مقايسه اثرات داروي مالتوفر و قند مالتوز بر ميزان رشد و مرفولوژي كلني برخي از درماتوفيت ها

Comparison of Maltofer And Maltose Effects on Growth And Morphology of Dermatophyte spp Colonies

Introduction: Dermatophytes are a group of fungi that cause infection to humans and animals. These fungi have different species. In view of the effects of Maltofer and Maltose, varieties of them should be selected to represent all the different types of dermatophytes. Therefore, Microsporum gypseum from soil Microsporums, Epidermophyton fluccosom from human Epidermophytons, Trichophyton rubrum from human Trichophytons and Microsporum canis from animal Microsporums were chosen as representatives. Materials and Methods: In this research, Maltofer (consisting of Maltose and Fe III as components) in 50,100, 200, 500, 1000, 1500 and 2000 mg / L concentrations and Maltose in 500, 1000 and 2000 mg / L concentrations were separately added to SCC culture medium. Then, the fungi were suspended in normal saline and mycelia segments and spores were counted in WBC count part of Neubauer slide, so that each 50 fiL of fungal suspension contained 1000 mycelium segments and fungi spores. Then, in the center of each different concentration of culture media and also control culture, wells with equal diameters were created to which both Maltose and Maltofer groups of 50 jiL of fungal suspension were injected. After inoculation, all the culture media were set in the same temperature and moisture conditions for two weeks. In the first and second weeks, the diameter of each colony was evaluated and measured. Findings: In evaluation of fungal colony area after 1 and 2 weeks, it was observed that all the four above mentioned fungal varieties in both Maltofer and Maltose groups showed more growth than the control. The fungi inoculated in Maltofer containing media showed more growth and irregularity in different concentrations while the growth of all fungi in Maltose containing media was lesser and more uniform, so the more increased Maltose concentration the more increased the diameter of colonies. Discussion and Conclusion: According to the finds, it can be deduced that Maltose a nutrient for the fungi, increase in its concentration causes increase in fungal growth, but not with an escalating trend. So, it is assumed that dermatophytes just absorb Maltose up to their level of requirement. Maltofer contains iron, i.e. a microelement of fungi with different effects in various concentrations on dermatophyte growth compared to control and Maltose containing media. Regarding presence of Maltose in Maltofer and culture media containing Maltose, increased growth in Maltofer containing media may be related to iron III presence in them. q\)CSuAfc q OAibCuj )\ ^isp Q \UJ) oVjxa M \iS 9 ^SN^DLA tfqjb 0\p\ fUxulILa Comparison of Maltofer And Maltose Effects on Growth And Morphology of Dermatophyte spp Colonies Ebrahimian Sh1*, Mikaeili A2, Zeini F3, Rezaei S3, Safari Sh4, Maleki A4 (Received: 6 Nov. 2009 Accepted: 16 sep. 2010) Keywords: maltofer, dermatophytes, maltose