بررسي تست سرطان زايي (Ames assay) در آزمايشگاه هاي پزشكي قانوني تهران و مقايسه آن با آزمايشگاه هاي پاتولوژي دانشگاه علوم پزشكي تهران

The study of carcinogenicity test (Ames test) in forensic medicine laboratories of Tehran and comparison with Tehran university medical sciences laboratories

Background: cancer is one of the most frequent disease in the world and mutation has an important role in etiology of cancer (approximately 90%of mutagens are carcinogen). Therefore Lab assistances that are working with mutagenic agents such as: Benzene , Xylazine and Formaldehyde for long period of time may be exposed to overload of these carcinogens. In Previous study, Muatgenicity test on urine sample of laboratory pathology technician of Tehran university medical sciences determined mutagenic agents activity. In regards to results, Mutagenicity assay was considered on forensic medicine laboratory of Tehran. Methods: Urine samples were collected at the end of the work shift and then were extracted by cl8 column for the maximum concentration of pollutants . Then extracts were tested by TA100 and TA98 standard strains as well both with and without metabolic activator. Alireza Partoazar * Mohammad hasan Abedi2 Mahmod Ghazi Khansari3 Majidreza Basiri4 Mahdi Kaviani5 1- Ph.D student of Nanomedicine 2- MD, Forensic medicine specialist 3- Ph.D, Professor of Toxicology 4- MSC in toxicology 5- Bachelor of Biology Results: Levels of toxicity and mutagenicity were detected by TA98 strain with activator in two personnel of pathology lab . Correspondentʹs Address: Pharmacology dept., School of medicine, (T.U.M.S) Porsiona Street, Keshavarz Avenue, Tehran, Iran. Telfax: 66402569 ,09126066356 Conclusion: Our results shows that forensic medicane pathology lab. worker have the same risk of cancer as the TUMS pathology lab. personals. I loecause smoking and other confounding factoris not recorder false positive resulte is probebly diseregarded in this study. Key words: Ames test,Carcinogenicity, Forensic medicine, Pathology laboratory