تاثير pH، نسبت رقيق سازي، يون ها و اسمولاريته برروي حركت اسپرماتوزوآ در ماهي شيپAcipenser nudiventris Lovetzky، 1828)

Spermatozoa motility in the ship sturgeon {Acipenser nudiventris Lovetzky, 1828): Effects of pH, dilution ratio, ions and osmolality

In this study effects of extenders on motility of spermatozoa (pooled semens from 3 male) of the ship sturgeon (Acipenser nudiventris) were investigated. Optimum ionic content (Na+, K+, Ca2+ and Mg2+), pH and buffer content (tris, tris-hcl, hepes) of activation solution as well as the optimum dilution rate and osmolality were determined. The results show optimum motility characteristics of spermatozoa in buffered solutions tris-hcl 30 mML"1 containing 12.5, 1, 7 and 15 mML-1 Na+, K+, Ca2+ and Mg2+, respectively, at dilution rate 1:100 and pH 8.5. At osmolalities of 68-94 mOsmol Kg"1 the spermatozoa motility was partly inhibited but the motility of A. nudiventris spermatozoa is inhibited by high osmotic pressure (133 mOsmol Kg"1 or more). The results of the present study demonstrated that various parameters such as ion concentration (Na+, K+, Ca2+ and Mg2+), osmotic pressure, pH, buffer content and dilution rate, affect the motility duration of ship sturgeon spermatozoa. K+ concentration in combination with osmotic pressure controlled spermatozoa motility in ship sturgeon. In conclusion Na+, Ca2+ and Mg2+ counter the inhibitory action of K+ and bivalent cations (Ca2+ and Mg2+) are more effective than Na+.