تاثير القاي تريپلوئيدي بر برخي شاخصهاي خون شناسي ماهيان نابالغ تمام ماده قزل آلاي رنگين كمان ( oncorhynchus mykiss)

Effect of Triploidy Induction on Some Hematological Indices in Premature All-female Rainbow Trout (Oncorhynchus my kiss)

Effect of triploidy induction on some hematological indices of premature all-female diploid and triploid rainbow trout, Oncorhynchus my kiss, was investigated in winter season. All female diploids were obtained by insemination of spermatozoa from sex reversed females (Neomale) with oocyte of normal females. All-female triploids were also produced similarly and by using heat shock of 26.5°C for 20 minutes to eggs 20 minutes post fertilization. Blood samples were taken from fish caudal vein using 5% EDTA as anticoagulant. Hematological data indicated that in all female triploid, mean number of total erythrocytes, hematocrite value and hemoglobin content were significantly lower (p<0.01) than all female diploids while mean corpuscular volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration were significantly higher (p<0.05) than all- female diploids. Furthermore, the mean number of total leukocytes was significantly lower in triploids (p<0.01), but there was no significant difference in differential counts between lymphocyte and neutrophile percentage in diploids and triploids (p>0.05). Although erythrocyte hematological indices were lower in triploids, but increased mean corpuscular volume and consequently higher mean hemoglobin concentration have set the equivalency between hematological indices in all-female triploids.