توليد كمپلكس 61Cu - بلئومايسين به عنوان يك راديوداروي احتمالي تومور گرافي گسيل پوزيترون (PET) و بررسي زيستي آن در موش هاي سالم و توموري

Preparation of [61Cu]Bleomycin Complex as a Potential PET Radiopharmaceutical and itʹs Biological Evaluation in Normal and Tumor-Bearing Rodents

[61Cu]Bleomycin ([61Cu]BLM) was prepared using [61Cu]CuC12 produced via natZn(p,x)61Cu (180jiA proton irradiation, 22MeV, 3.2h), purified by ion chromatography method. [61Cu]BLM was prepared at optimized conditions (room temperature, 45 mill, 0.1 mg bleomycin for 2-10mCi 61CuC12) with radiochemical purity over 98% determined by HPLC and RTLC. [6lCu]BLM was administered into the normal and tumor bearing rodents up to 210 minutes followed by biodistribution and co incidence imaging studies. A significant tumor/non tumor accumulation was observed either by animal sacrification or imaging method. [61Cu]BLM can be a potential PET radiotracer for tumor imaging.