توليد لايه نازك كامپوزيت كراتين -كايتوسن- ژلاتين و بررسي خواص مكانيكي و چسبندگي سلولي آن

Preparation of A Keratin - Chitosan - Gelatin Composite Film and Evaluations of Mechanical Properties and Cell Adhesion to Use in Lens and Implant

Keratin film without any additive is too fragile, 10-30 wt% of chitosan addition gave strong and flexible film (ultimate strength: 27-34 MPa, ultimate elongation: 4- 9%). Glycerol (20 wt%) also afforded flexibility to keratin film (ultimate strength: 1 MPa, ultimate elongation: 28%). Further addition of chitosan to glycerol-containing keratin film increased the ultimate strength to 9- 14 MPa but gave little effect on ultimate elongation. Keratin - chitosan composite film was prepared by casting the mixed solution of both biopolymers in 75% acetic acid. Waterproof characteristics such as swelling behavior and mechanical properties after swelling were much ameliorated for the composite film compared with keratin-chitosan-gelatin films, respectively. Chitosan/gelatin composite films are more permeable, transparent, flexible and biocompatible films and could potently be used for contact lens material. These data suggest that mechanical properties of keratin film are adjustable by appropriately adding chitosan and glycerol.