اثر تنظيم كننده هاي رشد بر كالوس زايي دو رقم كلزا (Brassica napus L)

Effects of Growth Regulator on Callus Induction of two Rapeseed (Brassica napus L.) cultivars

To improve in vitro tissue culture of rapeseed, a factorial experiment, based on completely random design with 4 replications, was carried out at the Agricultural Research Station of Islamic Azad University, Tabriz branch, during the growing season of 2007. In this experiment the effects of various concentrations of 2,4-D(l and 2 mg/1) and kinetine (0,0.5,1 and 2 mg/1) on callus induction and vitrification of hypocotyl and cotyledon from explants of two cultivars (Modena and Okapi) were studied. The three way interaction of variety kinetine 2,4-D indicate that there was differences between treatments for callus induction. Highest callus induction (82.33%) was obtained from the medium containing 2mg/l 2,4-D and 0.5 mg/1 kin. in Okapi. Mean comparisons revealed that frequency of callus vitrification for Modena variety was higher than Okapi variety. Hypocotyls in the medium containing lmg/1 2,4-D produced highest vitrification percentage. Highest callus weight, in both cultivars, was obtained when lmg/1 2,4-D was used. Callus weight and its diameter in culture of cotyledon were greater than hypocotyl. Medium containing lmg/1 2,4-D concentration increased callus diameter significantly.