ارزيابي ژن cry1Ab در نسل هاي مختلف در حال تفكيك برنج

Evaluation of crylAb Gene in Different Segregating Populations of Rice

Through an application of genetic engineering, genes from a soil bacterium Bacillus thuringiensis (Bt) which confer resistance to pests were transferred into rice. Successful employment of genetic engineering in traditional breeding programs is dependent upon stable transmission of transgene in transgenic varieties. Three different segregating F2 populations were developed from a cross between each one of the transgenic lines namely: Tarom Molaii, Neda and Nemat and Sang Tarom while the segregation mode of crylAb gene being studied in these populations. The results of phenotypic assay in field and genotypic analysis (employing PCR in laboratory on the different F2 populations) revealed monogenic segregation ratio for crylAb gene. The results indicated single copy integration of crylAb gene in the genomes of transgenic Tarom molaii, Neda and Nemat. This illustrates predictable segregation pattern of crylAb gene in transgenic plants. In addition, the data obtained imply that integration of crylAb gene in the genome of the studied transgenic lines is stable and can be successfully transmitted to successive generations. So, these transgenic lines could be well employed for improving pest tolerance of varieties in integrated pest management programs. E-mail: ghkiani@gmail.com Keywords: Rice, crylAb, Segregation, Insect resistance