پديد آورندگان :
ديلمي خياباني، زهرا نويسنده دانشگاه آزاد اسلامي واحد علوم و تحقيقات تهران Deilami Khiabani , Zahra , نجم آبادي، حسين نويسنده Najmabadi, H , كهريزي ، كيميا نويسنده Kahrizi, K , جوادي، غلامرضا نويسنده javadi, gholamreza , قره سوران، جلال نويسنده مركز تحقيقات ژنتيك-دانشگاه علوم بهزيستس و توانبخشي , , اصغريان، علي محمد نويسنده گروه زيست شناسي-دانشگاه آزاد اسلامي واحد علوم تحقيقات تهران Asghariyan, A , بنان، مهدي نويسنده مركز تحقيقات ژنتيك-دانشگاه علوم بهزيستي و توانبخشي Banan, M
چكيده لاتين :
Background: CREB is an important downstream protein for many signaling pathways. By having efficient siRNAs against CREB, it may be possible to assess the role of molecules involved in signaling pathways in different cell types. In this research the efficiency of CREB knockdown by 2 different siRNAs in HeLa cells has been studied. Materials and methods: HeLa cells were transfected by siRNAs using Lipofectamine 2000. RNA extraction and cDNA synthesis have been done. The efficiency of CREB knockdown has been assessed by quantitative relative real time PCR. According to our results only one of the siRNAs has a high level of inhibitory effect on CREB gene expression in HeLa cells. The expression of CREB by this siRNA was knocked-down by 64.4% in HeLa cells. Conclusion: By using efficient siRNA specifically for knocking down of CREB gene, we can apply this efficient siRNA in different cell types due to study of signaling pathway which CREB may be involved.
