بررسي اثر تزريق داخل صفاتي كلونيدين بر علائم قطع مصرف مرفين در موش هاي صحرائي نر وابسته به مرفين

Clonidine injection (i.p.) affect withdrawal syndrome in morphine dependent male rats

Adrenergic neurotransmission plays important role in opiate withdrawal. In this study the effect of alpha-2 agonist (clonidine) on opiate withdrawal were studied because there are opposite reports about its actions. Animals divided to 4 groups: Control and three groups that they received clonidine in three doses (0.02 mg /kg, 0.2 mg/kg and 2 mg/kg). Animals were addicted by injection of morphine and withdrawal syndrome was induced with injection of 20mg/kg (i.p.) naloxone. Withdrawal signs were evaluated for 30 min. Clonidine had not affected grooming, paw tremor and ejaculation and other sign such as body tremor in doses. 0.02 mg/kg (P<0.02), 0.2 mg/kg (P<0.04) and 2 mg/kg (P<0.002), defecating in doses 0.02 mg/kg (P<0.015), 0.2 mg/kg (P<0.039) and 2 mg/kg (P<0.015), chewing in doses 0.02 mg/kg (P<0.0001), 0.2 mg/kg (P<0.001) and 2 mg/kg(P<0.001), teeth chattering in doses 0.0 2mg/kg (P<0.02), 0.2 mg/kg( P<0.01) and 2 mg/kg P<0.02) and wet dog shakes in doses. 0.02 mg/kg (P<0.026), 0.2 mg/kg (P<0.011) and 2 mg/kg(P<0.034) decreased significantly. This drug increased standing on feet (P<0.02)and jumping (P<0.005) in 2 mg/kg dose. It seems that clonidine decreased signs by induce endogenous opiate release or affects alpha-2 receptors and inhibit noradrenalin release Enhancement of signs may be related to clonidine action on the other receptors such as al-adrenoceptors or imidazoline receptors.