بررسى اثر تزريق زير پوستى مايع مغزى نخاعى برميزان دژنراسيون موتونورون هاى نخاع پس از كمپرسيون عصب سياتيك در رت

The effect of subcutaneous injection of cerebrospinal fluid on alpha motoneuron degeneration after sciatic nerve injury in rats

Demolition of alpha neuron cell bodies in spinal cord that their axon was destroyed along with PNS damages is considered as an irreversible damage. This fact that Cerebrospinal Fluid (CSF) influences the viability and reproduction of neuron had led us toward applying that in living bodies. CSF consists of valuable components for protection of nervous systems. In this study, 18 adult male and 18 pregnant female (20-days after pregnancy) Wistar rats with average body weight of 200 to 250gr were used for the tests. The male rats divided randomly to 3 groups of control, compression, com+CSF injection (N=6). In compression group, the right leg sciatic nerve get through compression (60sec); in control group, the muscle surrounding the sciatic nerve was cut without any harm to the nerve; and in CSF injection group, in addition to compression, 10Ml of CSF was injected every 3 days and then we make use of 20-day germs in order to extract CSF. After 4 weeks of post-operations in which lumbar segments of spinal cord were sampled, processed, serially sectioned and stained with toluidine blue (Ph=4.65) the member of alpha motoneurons were counted using stereological quantitative technique and compared with each other. Statistical analyses revealed a remarkable increase (p<0.05) in CSF groups. The results indicated that CSF have neu-ronprotective effects on nerves system and inhibit the degeneration of cell body after sciatic nerve injury.