بررسي انتروتوكسين زائي استرين هاي متحرك آئروموناس جداشده از ماهي هاي قزل آلا با استفاده از روش لوپ ايلئال خرگوش (RIL)

Enterotoxin Production by Motile Aeromonas Sterain Isolated from Trout in Fish Stores of Zanjan Using Rabbit illeal Loop Method

In recent years, world wide reports of isolation of motile Aeromonas species from patients with gasterointestinal diseases and diarrhea ill have been increased. The main purpose of present study is the assessment of motile Aeromonas and other bacteria and assey of entrotoxin production. After isolation of various species of Aeromonas, using differential media and biochemical testes, Enterotoxin production was assesed by( Rabbit illeal Loop Method )RIL technique. The cell free supernatants from various bacterial cultures were prepared by transporting some colonies from blood- agar medium to TSB medium containing glucose and yeast extracts and then putting it in 37 c agitating water bath for 18-24 hours. RIL technique was performed following anesthesizing animals and creating loops of 5cm length in the ileum followed by injection of lml supernatant to each loop. One loop was injected only with pure culture medium as a negative control. After 18 hours the animals were killed and the excess fluid secretion of various loops were compared with that of control loop. The results demonstrated that from 110 fish samples, prevalence of bacteria was as follows: motile Aeromonas 123 colony, vibrio species 20 colony and other bacteria 36 colony. In this expermental study 65.85% species of Aeromonas were able to produce entrotoxin.