طراحي كيت الايزاي غير مستقيم جهت تشخيص بروسلوزيس در انسان

Designing of an Indirect ELISA Kit for the Detection of Brucellose in Human

Brucellosis is a zoonotic disease that is endemic in Iran. The appropriate and rapid diagnosis of brucellosis has a critical role in public health improvement. Introducing immunoassys in brucella diagnosis require a comparative study with reference techniques such as the standard agglutination test (SAT), Wright test, Complement fixation (CFT) and 2-mercaptoethanol test (2-ME).In Iran, the standard agglutination test (SAT) is the officially screening test. The aim of this study is to design an indirect ELISA in serological diagnosis of brucellosis with higher sensitivity and specificity than most commonly used conventional techniques. In this study, we used Brucella melitensis smooth lipo polysaccharid (S-LPS) for coating ELISA micro plate and combination of three different secondry conjugates to achieve better results. To assess our designed ELISA, 194 serum samples obtained from medical diagnostic laboratories were used. Among 119 positive sample (done by wright test) all serums had same results and our designed ELISA showed sensitivity of 100%. Among 75 samples that have been reported negative by rapid agglutination and the standard agglutination test we obtained negative response for 72 serums which showed specificity of 96%. Therefore, cut off was determined 0.19. The higher accuracy and speed are advantages of our designed kit in comparison with other serological tests and the commercial kits.