بررسي ارتباط پلي مورفيسم هاي ژن مانوز متصل به لكتين با بهبودي بيماران مبتلا به هپاتيت مزمن B

Studying of mannose binding lectin (MBL) gene polymorphisms among patients with chronic HBV and spontaneously recovered subjects

Background: Mannose-binding lectin (MBL) is a constituent of the human innate immune system which may play an important role in combating a variety of infectious diseases and thus may be important for determining hepatitis B virus (HBV) persistence. In this study, we determined MBL genotypes in chronic hepatitis B subjects, spontaneously recovered subjects and healthy controls. Methods: In a case-control study, we examined 100 unrelated patients with chronic hepatitis B and 100 spontaneously recovered patients were referred to Taleghani Hospital and 100 healthy controls which all had been matched by sex and age. Genomic DNA was extracted from blood samples and the following genetic polymorphisms were studied by PCR-RFLP methods: two point mutations in the promoter region at position -550(H/L variants),-221 (X/Y variants), one point mutation in the 5ʹ untranslated (UT) region at position +4(P/Q variants) and three point mutations located at codon 52, 54 and 57 in exon 1 of the MBL gene, at nucleotide position 223,230 and 239, repectively. Result: Our finding has shown that the frequency of +223 T allele of Codon 52 in chronic HBV patients was 0/22 and 0/37 in spontaneous recovered subjects, respectively. Our results show that there is no significant association between each polymorphisms of the MBL gene with persistent HBV infection in Studying population excepting codon +52 (p value=0.0). Conclusion: Heterozygous for codon 52 mutant allele (C/T) may be associated with recovery from chronic hepatitis B.