شناسايي گونه هاي مالاسزياي جداشده از بيماران مبتلا به پيتيريازيس ورسيكالر با استفاده از روش PCR-RFLP

Identification of Malassezia species associated with pityriasis versicolor using PCR-RFLP

Background and Aim: Malassezia is a lipophilic and dimorphic fungus which has different species. Some of them can be found as natural flora on skin and in some conditions may cause pityriasis versicolor. The aim of this study was to identify Malassezia species associated with pityriasis versicolor in Iranian patients, using PCR-RFLP. Methods: In this study out of 65 patients with pityriasis versicolor to have pityriasis versicolor,isolates of 60 patients were positive. Malassezia species, using by PCR-RFLP. The Internal Transcribed Spacer 2 (ITS2) region was amplified by PCR employing the ITS3 and ITS4 primers and The restriction endonucleases Alul, Banl and MspPd were selected for producing distinct RFLP patterns. Results: M. furfur (36.7%), M globosa (30.0%), M. sympodialis (20.0%), M. slooffiae (8.3%), M. restricta (3.3%) and M. obtusa (1.7%) were the microorganisms responsible for the infection among participants. The M. sympodialis infection was strongly correlated with the female gender (P=0.02). Conclusion: Our findings suggest that, the most common Malassezia species associated with pityriasis versicolor was M. furfur, followed by M. globosa. Key words: Malassezia; pityriasis versicolor; PCR-RFLP Received: Feb 12, 2011 Accepted: May 22, 2011 Dermatology