ارزيابي تاثير مهار كننده ي باسيلوس ليكنيفورميس بر آفلاتوكسينهاي B1 و B2 در محيط كشت آزمايشگاهي و آرد ماهي كيلكا

Inhibitory Effect of Bacillus licheniformis on B1 and B2 Aflatoxins in Culture Media and Kilka Fish Meal

In this study, inhibitory effect of Bacillus licheniformis on B1 and B2 aflatoxins was examined in two scales (culture media and Kilka fish meal).In culture media, two concentrations of aflatoxins (12 and 16 ppb for B1, 8 and 12 ppb for B2) and two doses of the bacillus (3 and 4%)were used. In fish meal, were also two concentrations of aflatoxins (2 and 4 ml from aflatoxins standard/ 50g of fish meal). Concentration of bacillus was similar to culture media. Changes of aflatoxins and the bacterium growth were evaluated by HPLC and spectrophotometer (OD: 600 λ) respectively. Culture media results were showed that B1 and B2 concentrations have been decreased to 88.6 and 84.7%, 98.1 and 92.4% respectively (for 3% Bacillus) and 95.2 and 90.6%, 99.1 and 97.3% respectively (for 4% Bacillus). In fish meal, concentrations of B1 and B2 have been decreased to 76.5 and 70.6% (for 3% Bacillus) and 96.9 and 80.4% (for 4% Bacillus) respectively. The conclusion showed that Bacillus licheniformis is able to significantly decrease B1 and B2 aflatoxins in culture media and meal and can be used as a biological tool in different feeds particularly fish meal.