بررسي نقش بيولوژيك عصاره ي زعفران در تحريك فعاليت ضد ميكروبي انتروكوكوس هايرائي

Evaluation of biological effect of Saffron extract on stimulation of Enterococcus hirae antimicrobial activity

The Object of this research was Evaluation of biological effect of Saffron(Crocus sativus) extract on stimulation of Enterococcus hirae antimicrobial activity. After the culture of Enterococcus hirae (PTCC 1239) in the BHI broth medium culture, centrifuging and isolation of microbial mass, antimicrobial compound of this strain was purified by dialysis and molecular weight was estimated by SDS-PAGE electrophoresis. Crocus sativus extract was combined with microbial suspension . Total activity, activity unit, Protein concenteration were determined in samples. Antimicrobial effect of these samples were evaluated throuh well diffusion method. Minimum inhibitory concentration of antimicrobial compounds was determined. Antimicrobial effect and inhibitory concentration of Enterocin were evaluated on Staphylococcus aureus and Bacillus cereus and Listeria monocytogenes and Klebsiella pneumonia. The most antimicrobial effect and the least inhibitory concentration produced by Enterocin was on Listeria monocytogenes. Concerning the results gained from this plant, Saffron extract can have synergistic effect on Enterocin production and antimicrobial effect of Enterococcus hirae .