بررسي ساخت فيلم هاي پليمري شفاف جهت استفاده از باكتريورودوپسين به عنوان نانوحافظه هاي پروتئيني

Preparation and Construction Survey of Transparent Polymeric Films for Utilization of Bacteriorhodopsin as Nano Memory of Protein.

Introduction: In the last few years Bacteriorhodopsin (bR) became probably one of the most studied proteins due to its high stability and a variety of properties which are suitable for electronics, optics and other applications. Speaking about applications, it is preferential to have bR in the form of films, as it is easier to manipulate with such a type of sample. In a design of a device based on bacteriorhodopsin, a matrix in which the active molecule is embedded is usually needed for ease of manufacture and to provide good optical quality, and is also very important for film stability. Aim: The influence of different organic and inorganic matrices (polyvinyl alcohol (PVA), gelatin, and silica) on the optical properties of bacteriorhodopsin (bR)- containing films were studied. Materials and Methods: The photosynthetic membrane protein of bacteriorhodopsin (bR) was encapsulated in optically transparent silica, PVA and GEL matrix and the absorption spectra bR were studied. Morphological studies of the bR-containing films using AFM. Results: In presence of laser rays, attraction extent is reduced in 586nm and maximum attraction is observed in 410nm. This venture is applied for storage of information. In three kinds of polymeric matrix, there is not observed salient changes in the maximum attraction wave length. The film concerning bacteriorhodopsin in Gel matrix and Gel & PVA matrix has a homogeneous and monotonous structure and the bacteriorhodopsin is distributed in the matrix diligently. The films concerning bacteriorhodopsin in Gel and PVA matrixes has higher transparent in proportion to the other matrixes. Conclusion: polymeric gelatin matrix and polyvinyl alcohol are applicable as suitable as station in nanomemory of protein, due to preservation of radioactivity bacteriorhodopsin, not changing in bacteriorhodopsin maximum attraction wave length, high transparent, homogeneous and monotonous structure, integrated distribution of bacteriorhodopsin and adequate diameter in nanometer quantity.