بررسي تاثير ديازينون بر پروتئوم گياه Aegilops columnaris Zhuk با استفاده از الكتروفورز دو بعدي

The Effects of Diazinon on Aegilops columnaris Zhuk Proteome Using Two-Dimensional Electrophoresis

Diazinon [O, O-Diethyl 0-(2-isopropyl l-6-methyl-4 Pryimidinyl) Phosphorothioate] is an organophosphorus pesticide (OPP) that is extensively used as pesticide in agriculture and some Aegilops species are considered as the progenitors for polyploidy Triticum L. species and cause the evolution of Triticum genus. Also two-dimensional gel electrophoresis (2- DE) is a powerful and widely used method for the analysis of complex protein mixtures extracted biological samples. These reasons caused effects of diazinon on Aegilops columnaris Zhuk proteome is studied using 2-DE. Five treatment pots were prepared: 1 (0 diazinon, control), 2 (0.01 diazinon, 1 time exposure), 3 (0.01 diazinon, 2 times exposure), 4 (0.001 diazinon, 1 time exposure) and 5 (0.001 diazinon, 2 times exposure). Ten Aegilops columnaris seeds were treated in each pot. Each treatment had 10 repetitions. After 2 weeks under equal condition in growth chamber all of samples except control were exposure with diazinon and after 3 weeks treatments 3 and 5 treatment received diazinon. After 24 h all of control and treated plants were prepared for isolating proteins using two-dimensional electrophoresis (2-DE) method. Results showed changes of total protein pattern in all treated dizinon plants in compared to control that changes depend on diazinon concentration and exposure times.